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Last verified: 17 days ago

Bacillus subtilis DE111

Also known as: B. subtilis DE111, DE111, spore-forming probiotic DE111

Evidence under review. — Not yet rated

Spore-forming probiotic with early evidence for gut health, immune support, and cardiovascular benefits.

  • What it does

    Bacillus subtilis DE111 is a spore-forming probiotic bacterium that survives the harsh environment of the digestive tract. Clinical studies suggest it supports gut microbiota balance, modulates...

  • Evidence quality

    Evidence base hasn't been formally rated yet. See research below.

  • Clinical dose

    1×10^9 CFU daily based on study doses

  • Found in

    IM8 Daily Ultimate Essentials, Amazing Grass Super Greens The Original

See full breakdown ↓ Methodology →

What the Science Says

Bacillus subtilis DE111 is a spore-forming probiotic bacterium that survives the harsh environment of the digestive tract. Clinical studies suggest it supports gut microbiota balance, modulates immune function, and may improve endothelial (blood vessel) function. Most studies used 1 billion CFU daily, with effects observed over 4–8 weeks.

What It Doesn't Do

Not proven to treat or prevent cardiovascular disease in humans. No strong evidence it helps with weight loss. Gut barrier improvements seen in animal studies didn't hold up in cell culture models. Don't expect dramatic results in a few days.

Evidence-Based Benefits

Supports a balanced gut microbiome and may reduce gastrointestinal discomfort.

Moderate Evidence

Effective at: 1×10^9 CFU daily

Supporting studies (click to view on PubMed):

PMID 33671071 PMID 37026366

Modulates immune system activity, particularly in children and healthy adults.

Moderate Evidence

Effective at: 1×10^9 CFU daily

Supporting studies (click to view on PubMed):

PMID 33671071 PMID 33161736

May improve blood vessel function, especially in people eating a high-fat Western diet.

Weak Evidence

Effective at: 1×10^9 CFU daily

Supporting studies (click to view on PubMed):

PMID 39631408 PMID 33161737

Early evidence suggests it may support heart health by improving gut microbiota linked to cardiovascular risk.

Weak Evidence

Effective at: 1×10^9 CFU daily

Supporting studies (click to view on PubMed):

PMID 33161737 PMID 39631408

Absorption & Bioavailability

Good — spore-forming bacteria survive stomach acid and germinate in the gut, giving DE111 a survival advantage over non-spore-forming probiotics.

Red Flags to Watch For

  • Most human trials are small and short-term; long-term safety data is limited
  • Cardiovascular and endothelial benefits are preliminary — mostly animal or small human studies
  • Over 1,000 products on the market contain DE111, but quality and CFU counts vary widely between brands
  • Mechanisms of action (e.g., how it protects blood vessels) are not yet fully understood

Products Containing Bacillus subtilis DE111

See how Bacillus subtilis DE111 is used in these analyzed products:

IM8 Daily Ultimate Essentials

Supplement

Overhyped

Amazing Grass Super Greens The Original

Supplement

Mostly Legit
View all product reviews →

Frequently Asked Questions

What does Bacillus subtilis DE111 do?

Spore-forming probiotic with early evidence for gut health, immune support, and cardiovascular benefits.

What is the effective dose of Bacillus subtilis DE111?

1×10^9 CFU daily based on study doses

Is Bacillus subtilis DE111 safe?

Most human trials are small and short-term; long-term safety data is limited

What doesn't Bacillus subtilis DE111 do?

Not proven to treat or prevent cardiovascular disease in humans.

Research Sources

  • PubMed
  • NIH DSLD

This information is for educational purposes only and is not medical advice. Always consult a healthcare professional before starting any supplement regimen. Last updated: 2026-05-25